Reliable QC analysis of mRNA and ribo-depleted total RNA is achieved with the Agilent Automated Electrophoresis portfolio. Sample quality assessment helps ensure the integrity of the mRNA of interest, and guarantees the sample contains minimal contaminating rRNA, which could interfere with robust downstream analysis. Quantitative applications for gene expression also rely on having intact mRNA, as degraded total RNA may lead to the absence of some transcripts, and misinterpretation of results. As with other NGS applications, proper mRNA QC analysis of nucleic acids ensures that only suitable samples are processed. The mRNA is then analyzed with an assay to verify that the residual rRNA content is low, as indicated by a low percent rRNA contamination score. In a typical workflow involving mRNA, total RNA is first analyzed for sample integrity before ribosomal RNA (rRNA) depletion, to ensure that the RNA is not degraded. Large-scale sequencing of mRNA enables researchers to profile what genes and genomic regions are active. Messenger RNA (mRNA) provides researchers with insight into gene activity. Reduce Contamination and Misinterpretation in Messenger RNA Support Services, Agreements & Training. Shorten the time it takes to start seeing the full value of your instrument investment Instrument & equipment deinstallation, transportation, and reinstallationĬrossLab Connect services use laboratory data to improve control and decision-makingĪdvance lab operations with lab-wide services, asset management, relocation Learn essential lab skills and enhance your workflows Instrument/software qualifications, consulting, and data integrity validations Software to manage instrument access, sample processing, inventories, and more Service Plans, On Demand Repair, Preventive Maintenance, and Service Center Repair Microscopes and Microplate Instrumentation.Chromatography & Spectroscopy Lab Supplies.
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